TE Buffer, Low EDTA, 1X, pH 8.0
TE Buffer, Low EDTA, 1X, pH 8.0
- Sterile, 0.20 µm filtered
- Molecular Biology Grade
- 10 mM Tris, 0.1 mM EDTA, pH 8.0
- RNase/DNase tested
- Endotoxin tested (≤ 0.10 EU/mL)
- 1X ready to use
UFC BIO
Cat no. / BTELE80-1X
In stock
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Certificate of Analysis (COA) Available
Safety Data Sheet (SDS) Available
Original Manufacturer
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Manufactured in New York, Made in the USA
Ready-to-use buffer for DNA and oligonucleotide resuspension, dilution and storage.
UFC BIO Low EDTA TE contains 10 mM Tris and 0.1 mM EDTA at pH 8.0: the same Tris concentration as standard 1X TE with one-tenth the EDTA.
Safety Data Sheet (SDS) and lot-specific Certificate of Analysis (COA) available.
Product Properties
| Catalog Number | BTELE80-1X |
| Formulation | 10 mM Tris, 0.1 mM EDTA |
| Concentration | 1X, ready to use |
| Grade | Molecular Biology Grade |
| pH at 25 °C | 8.0 ± 0.10 |
| Appearance | Clear, colorless liquid |
| Sterilization | 0.20 µm sterile filtered |
| Package Size | 500 mL |
| Storage | 4–25 °C |
| Intended Use | Laboratory, research and further manufacturing use only |
Product Highlights
- Lower EDTA carryover: 0.1 mM EDTA for workflows that specify low-EDTA TE.
- For resuspending and storing DNA oligonucleotides, including primers and probes, when recommended by the oligonucleotide supplier.
- Sterile, with a USP ⟨71⟩ sterility release specification; filtered through a 0.20 µm filter.
- RNase/DNase tested, with no activity detected as the release criterion.
- Endotoxin specification: ≤ 0.10 EU/mL.
- pH 8.0 ± 0.10, measured at 25 °C. Product of USA.
Common Applications
- Primer and probe stocks: Resuspend DNA oligonucleotides in low-EDTA TE when specified by the supplier.
- Purified DNA handling: Prepare plasmid or genomic DNA stocks and dilutions for protocols that accept 0.1 mM EDTA.
- PCR and qPCR sample preparation: Prepare compatible primer, probe or template stocks while reducing the EDTA introduced with the sample.
- Sequencing workflows: Resuspend or dilute DNA samples when the library preparation or sequencing protocol permits low-EDTA TE.
Why Choose Low EDTA TE
Tris buffers pH, while EDTA binds divalent metal ions to help limit metal-dependent nuclease activity. Lowering EDTA from 1 mM to 0.1 mM reduces the chelator introduced into downstream reactions at the same sample input volume.
EDTA also binds the magnesium many enzymes require. Follow your assay's buffer and sample-volume requirements; a lower EDTA concentration does not establish compatibility with every PCR, sequencing or enzyme reaction.
Preparation and Handling
Ready to use; no dilution is required. Store at 4–25 °C and use by the expiration date on the product label. Once DNA or oligonucleotides are added, follow the sample or assay supplier's storage instructions.
For laboratory, research and further manufacturing use only. Consult the Safety Data Sheet for handling information.
Frequently Asked Questions
How does this differ from standard 1X TE?
Both UFC BIO formulations contain 10 mM Tris. Low EDTA TE contains 0.1 mM EDTA; standard 1X TE contains 1 mM EDTA. Choose the formulation specified by your protocol.
Can I make it by diluting standard 1X TE tenfold?
No. That dilution would also reduce Tris to 1 mM. This product keeps 10 mM Tris while lowering EDTA to 0.1 mM.
How do I get the Certificate of Analysis?
Enter the Lot/Batch Number printed on the bottle in the COA search on the Documents tab.
| Test | Specification |
|---|---|
| Appearance | Clear, colorless liquid |
| pH at 25 °C | 8.0 ± 0.10 |
| Endotoxin | ≤ 0.10 EU/mL |
| DNase activity | No activity detected |
| RNase activity | No activity detected |
| Sterility | Complies with USP ⟨71⟩ |
| Sterilization | 0.20 µm sterile filtered |
| Product details | |
| Catalog number | BTELE80-1X |
| Grade | Molecular Biology Grade |
| Formulation | 10 mM Tris, 0.1 mM EDTA in 18.2 MΩ·cm deionized water |
| Preparation | Ready to use, no dilution required |
| Storage | 4–25 °C |
| Country of manufacture | USA |
Formulation values are nominal manufacturing targets, not analytical assay results.
Refer to the lot-specific Certificate of Analysis for actual results and specifications.
Shipping
Free standard ground shipping when your order includes $50 or more of UFC BIO products, shipped within the contiguous US (lower 48 states).
Returns
30-day returns within the contiguous US. Once we receive and inspect your return, we’ll issue a full refund within 3–5 business days.
Returns are not accepted more than 30 days after delivery unless an extension is approved.
Alaska, Hawaii, Puerto Rico & international
Orders shipping outside the contiguous US (including Canada) do not qualify for free shipping. Shipping costs are determined at checkout. If a shipping option isn’t available, please contact us at info@ufcbio.com.
Certificate of Analysis (COA)
Search for a Certificate of Analysis by entering the product’s Lot/Batch Number. You’ll find it on the product label after the word ‘Lot’ or ‘Batch’.
COA found. Opening the PDF in a new tab…
Safety Data Sheet (SDS)
Handling, storage and safety information for this product.
Download SDS (PDF) ↗