Dimethyl Sulfoxide (DMSO), ≥99.7%, Cell Culture Grade
Dimethyl Sulfoxide (DMSO), ≥99.7%, Cell Culture Grade
- Sterile, 0.20 µm filtered
- Assay ≥ 99.7% (GC)
- Non-animal, non-human origin
ufcbio
Cat no. / D67685-BIO-1PK
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Certificate of Analysis (COA) Available
Safety Data Sheet (SDS) Available
UFC BIO Cell Culture Grade DMSO (dimethyl sulfoxide), CAS 67-68-5, is a polar aprotic solvent for cell culture research, specified at ≥ 99.7% assay by GC on a dry weight basis.
It is 0.20 µm sterile filtered and tested for sterility to USP ⟨71⟩. Each lot is also controlled for identity by IR, water content, titratable acid and residue after evaporation.
Use DMSO as a component of cell freezing media or as a solvent for preparing research compound stocks. Select the final DMSO concentration and handling conditions for your cell type and experimental protocol.
Safety Data Sheet (SDS) and Certificate of Analysis (COA) available.
Product Information
| Chemical Name | Dimethyl sulfoxide |
| Catalog Number | D67685-BIO |
| CAS Number | 67-68-5 |
| Formula | (CH₃)₂SO |
| Molecular Weight | 78.13 g/mol |
| Grade | Cell Culture |
| Assay | ≥ 99.7% (GC, dry weight basis) |
| Identification, IR | Complies |
| Filtration | 0.20 µm sterile filtered |
| Appearance | Clear liquid |
| Package Sizes | 50 mL, 5 × 50 mL |
| Storage | 18 °C to 30 °C |
| Material Origin | Non-animal, non-human |
| Country of Origin | India |
| Expiration | See product label and lot COA |
Intended Use
- For R&D and further manufacturing use only. Not for human or veterinary use.
- Always review the Safety Data Sheet (SDS) before handling.
Key Features
- ≥ 99.7% assay by GC on dry weight basis
- 0.20 µm sterile filtered
- Sterility tested to USP ⟨71⟩
- Identity confirmed by IR
- Water ≤ 0.1% (Karl Fischer)
- Residue after evaporation ≤ 0.01%
- Non-animal, non-human origin
- Lot-specific COA and SDS available
Common Applications
- Cell cryopreservation: a cryoprotectant component for preparing cell freezing media according to a cell-specific protocol.
- Compound stock preparation: a solvent for dissolving compatible compounds used in cell-based assays and screening studies.
- Reagent preparation: solution preparation where DMSO is specified by the experimental method.
Storage and Handling
Store at 18 °C to 30 °C with the container tightly closed. DMSO is hygroscopic, so minimize exposure to moisture. Keep away from heat and ignition sources, and follow the SDS for handling and protective equipment.
DMSO freezes at about 18.5 °C (65 °F), so it may crystallize or solidify in cool storage or during shipping. This is a normal physical property and does not by itself indicate degradation. Let the closed container return to room temperature until fully liquid and mix gently before use. Do not microwave. Use aseptic technique when dispensing for cell culture work.
Quality Documentation
Each lot is supported by a Certificate of Analysis (COA) with the specifications and actual lot results. Enter the Lot/Batch Number printed on the product label in the COA search on the Documents tab. If your certificate is not available online, contact info@ufcbio.com. The Safety Data Sheet (SDS) is available on the same tab.
Learn more about UFC BIO Quality.
Frequently Asked Questions
How does this differ from ACS, USP, NF grade DMSO?
D67685-BIO is the cell culture grade: it is 0.20 µm sterile filtered and tested for sterility to USP ⟨71⟩. The separate ACS, USP, NF grade DMSO has its own chemical specifications. Choose the grade documented for your application.
Can I use it to prepare cell freezing medium?
DMSO is commonly used as a cryoprotectant component in cell freezing media. This product is concentrated DMSO; prepare the freezing medium according to your cell line's recommended protocol. The appropriate concentration and freezing procedure depend on the cell type.
Is it sterile?
Yes. The product is 0.20 µm sterile filtered and each lot is tested for sterility according to USP ⟨71⟩. Use aseptic technique once the bottle is opened.
Why has my DMSO solidified?
DMSO freezes at about 18.5 °C, close to room temperature, so it can crystallize in cool conditions. Let the closed bottle warm to room temperature until fully liquid before dispensing.
Is this material of animal origin?
No. The material is of non-animal, non-human origin.
What is the shelf life?
Use the expiration date printed on the product label and the COA for your lot.
Which sizes are available?
A single 50 mL bottle or a pack of five 50 mL bottles.
Learn more about DMSO properties, crystallization and storage in What Is DMSO (Dimethyl Sulfoxide)?
CAS Number 67-68-5
| Test | Specification |
|---|---|
| Chemical and Physical | |
| Appearance, Clarity | Clear liquid |
| Identification, IR | Complies |
| Assay, GC, on dry weight basis | ≥ 99.7% |
| Water, KF, colorimetric | ≤ 0.1% |
| Titratable acid | ≤ 0.001 meq/g |
| Residue after evaporation | ≤ 0.01% |
| Biological | |
| Sterility | Complies with USP ⟨71⟩ |
| General | |
| Filtration | 0.20 µm sterile filtered |
| Material origin | Non-animal, non-human |
| Storage | 18 °C to 30 °C |
| Country of origin | India |
| Intended use | R&D and further manufacturing use only |
Refer to the lot-specific Certificate of Analysis for actual results and specifications.
Published research that names UFC BIO DMSO (dimethyl sulfoxide) in its methods or materials. The papers do not state which grade was used.
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Cynaropicrin inhibits pancreatic cancer cell viability and disrupts cellular redox homeostasis
Leek HR, Oberhelman AL, Rew SR, Janss OM, Davidson CD. microPublication Biology. 2026.
Used at 0.05% in the control and treatment media for pancreatic cancer cell viability assays: “DMSO: UFC Biotechnology, Buffalo NY, USA.”
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Iron Oxide Mesoporous Magnetic Nanostructures with High Surface Area for Enhanced and Selective Drug Delivery to Metastatic Cancer Cells
El-Boubbou K, Ali R, Al-Humaid S, Alhallaj A, Lemine OM, Boudjelal M, AlKushi A. Pharmaceutics. 2021;13(4):553.
Listed among the materials for preparing doxorubicin-loaded nanostructures tested on cancer cells: dimethyl sulfoxide (DMSO) “purchased from UFC Biotechnology.”
See more studies in UFC BIO in Published Research.
Shipping
Free standard ground shipping when your order includes $50 or more of UFC BIO products, shipped within the contiguous US (lower 48 states).
Returns
30-day returns within the contiguous US. Once we receive and inspect your return, we’ll issue a full refund within 3–5 business days.
Returns are not accepted more than 30 days after delivery unless an extension is approved.
Alaska, Hawaii, Puerto Rico & international
Orders shipping outside the contiguous US (including Canada) do not qualify for free shipping. Shipping costs are determined at checkout. If a shipping option isn’t available, please contact us at info@ufcbio.com.
Certificate of Analysis (COA)
Search for a Certificate of Analysis by entering the product’s Lot/Batch Number. You’ll find it on the product label after the word ‘Lot’ or ‘Batch’.
COA found. Opening the PDF in a new tab…
Safety Data Sheet (SDS)
Handling, storage and safety information for this product.
Download SDS (PDF) ↗