
20X TBE (Tris-Borate-EDTA) Buffer is a concentrated stock solution of a zwitterionic buffering system used for polyacrylamide and agarose gel electrophoresis. It is diluted 1:20 to a 1X working concentration of 90 mM Tris-Borate and 2 mM EDTA, prepared with 18.2 Ω water and adjusted to pH 8.1 ± 0.1 at 24°C, providing stable pH control and chelation to protect against nuclease degradation.
Safety Data Sheet (SDS) and Certificate of Analysis (COA) - available
- Expiration date: 2 years from manufacturing date
100% Completely Sterile:
Commonly used Applications:
- Running buffer for separating DNA/RNA fragments
- Provides high-resolution separation of small DNA fragments in polyacrylamide sequencing setups
- Nuclease Protection
CAUTION: Research use only.
Product Properties
Property | Value |
---|---|
pH | 8.1 |
Form | Liquid |
Storage Temperature | Room temperature |
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