{"product_id":"pbs-phosphate-buffered-saline","title":"PBS (Phosphate Buffered Saline), pH 7.4, 1X \u0026 10X","description":"\u003cdiv class=\"description-content ufc-pdp\"\u003e\n\n  \u003cdiv class=\"ufc-pdp__intro\"\u003e\n    \u003cp class=\"ufc-pdp__lead\"\u003eUFC BIO Phosphate Buffered Saline (PBS), pH 7.4, is a liquid buffer for laboratory washing, sample preparation and cell resuspension where PBS is specified by your protocol.\u003c\/p\u003e\n    \u003cp\u003eChoose PBS 1X for use directly from the bottle. Choose PBS 10X when you prefer to prepare a 1X working solution as needed.\u003c\/p\u003e\n    \u003cp\u003eCited in published research, including 2025 studies in \u003cem\u003eCurrent Biology\u003c\/em\u003e and \u003cem\u003eiScience\u003c\/em\u003e. See the Citations tab.\u003c\/p\u003e\n    \u003cp class=\"ufc-pdp__note\"\u003eSafety Data Sheet (SDS) for each concentration and lot-specific Certificate of Analysis (COA) available.\u003c\/p\u003e\n  \u003c\/div\u003e\n\n  \u003cdiv class=\"ufc-pdp__aside\"\u003e\n    \u003cdiv class=\"table-container-properties ufc-pdp__comp\"\u003e\n      \u003ch3 class=\"bold-heading\"\u003eProduct Properties\u003c\/h3\u003e\n      \u003ctable class=\"properties-table\"\u003e\n        \u003ctbody\u003e\n          \u003ctr\u003e\n\u003ctd\u003eFormulation (at 1X)\u003c\/td\u003e\n\u003ctd\u003e137 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003epH at 25 °C (at 1X)\u003c\/td\u003e\n\u003ctd\u003e7.40 ± 0.05\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003eCalcium and Magnesium\u003c\/td\u003e\n\u003ctd\u003eNone added\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003eAppearance\u003c\/td\u003e\n\u003ctd\u003eClear, colorless liquid\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003eSterilization\u003c\/td\u003e\n\u003ctd\u003e0.05 µm filtered\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003eConcentrations\u003c\/td\u003e\n\u003ctd\u003e1X, 10X\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003eBottle Volume\u003c\/td\u003e\n\u003ctd\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e1X: 4–25 °C\u003cbr\u003e10X: 15–25 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003e2 years from manufacture\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003eIntended Use\u003c\/td\u003e\n\u003ctd\u003eLaboratory, research and further manufacturing use only\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003c\/tbody\u003e\n      \u003c\/table\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003cdiv class=\"ufc-pdp__main\"\u003e\n    \u003cdiv class=\"ufc-pdp__section\"\u003e\n      \u003ch3 class=\"bold-heading\"\u003e1X or 10X\u003c\/h3\u003e\n      \u003ctable class=\"ufc-pdp__compare\"\u003e\n        \u003cthead\u003e\n          \u003ctr\u003e\n\u003cth\u003e\u003c\/th\u003e\n\u003cth\u003ePBS 7.4 1X\u003c\/th\u003e\n\u003cth\u003ePBS 7.4 10X\u003c\/th\u003e\n\u003c\/tr\u003e\n        \u003c\/thead\u003e\n        \u003ctbody\u003e\n          \u003ctr\u003e\n\u003ctd\u003eSupplied as\u003c\/td\u003e\n\u003ctd\u003eReady to use\u003c\/td\u003e\n\u003ctd\u003eConcentrated stock\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003eCatalog Number\u003c\/td\u003e\n\u003ctd\u003eBPBS74-1X\u003c\/td\u003e\n\u003ctd\u003eBPBS74-10X\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003ePreparation\u003c\/td\u003e\n\u003ctd\u003eNo dilution required\u003c\/td\u003e\n\u003ctd\u003e1 part concentrate + 9 parts water\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003eVolume at 1X\u003c\/td\u003e\n\u003ctd\u003e500 mL\u003c\/td\u003e\n\u003ctd\u003e5 L\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e4–25 °C\u003c\/td\u003e\n\u003ctd\u003e15–25 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003c\/tbody\u003e\n      \u003c\/table\u003e\n    \u003c\/div\u003e\n\n    \u003cdiv class=\"ufc-pdp__section\"\u003e\n      \u003ch3 class=\"bold-heading\"\u003ePreparation\u003c\/h3\u003e\n      \u003cul\u003e\n        \u003cli\u003e\n\u003cstrong\u003ePBS 7.4 1X:\u003c\/strong\u003e Use directly. No dilution is required.\u003c\/li\u003e\n        \u003cli\u003e\n\u003cstrong\u003ePBS 7.4 10X:\u003c\/strong\u003e Dilute one volume of concentrate with nine volumes of suitable laboratory-grade water. To prepare 1 L of PBS 1X, combine 100 mL of PBS 10X with 900 mL of water.\u003c\/li\u003e\n        \u003cli\u003eUse sterile water and aseptic handling when your application requires sterility.\u003c\/li\u003e\n        \u003cli\u003eStore PBS 10X at 15–25 °C. If crystals form, warm to room temperature and mix until fully dissolved before diluting.\u003c\/li\u003e\n      \u003c\/ul\u003e\n    \u003c\/div\u003e\n\n    \u003cdiv class=\"ufc-pdp__section\"\u003e\n      \u003ch3 class=\"bold-heading\"\u003eCommon Applications\u003c\/h3\u003e\n      \u003cul\u003e\n        \u003cli\u003eWashing and resuspending cells\u003c\/li\u003e\n        \u003cli\u003ePreparing and diluting biological samples\u003c\/li\u003e\n        \u003cli\u003eWashing samples during flow cytometry preparation\u003c\/li\u003e\n        \u003cli\u003ePreparing buffers for immunostaining and microscopy\u003c\/li\u003e\n        \u003cli\u003eSupporting protein handling and biochemical assays where PBS is specified\u003c\/li\u003e\n        \u003cli\u003ePreparing PBS-based wash solutions for molecular biology workflows\u003c\/li\u003e\n      \u003c\/ul\u003e\n      \u003cp\u003eSelect the concentration and preparation method appropriate to your protocol. Confirm compatibility with your cells, samples and assay requirements.\u003c\/p\u003e\n      \u003cp\u003e\u003cstrong\u003eFor laboratory, research and further manufacturing use only. Not for diagnostic or therapeutic use.\u003c\/strong\u003e\u003c\/p\u003e\n    \u003c\/div\u003e\n\n    \u003cdiv class=\"ufc-pdp__section\"\u003e\n      \u003ch3 class=\"bold-heading\"\u003eQuality Testing\u003c\/h3\u003e\n      \u003cul\u003e\n        \u003cli\u003eEach lot is tested for appearance, pH, conductivity, endotoxin (≤ 0.10 EU\/mL), DNase and RNase activity, and sterility to USP ⟨71⟩.\u003c\/li\u003e\n        \u003cli\u003eFor PBS 10X, appearance is checked on the concentrate and all other tests are performed after dilution to 1X. Full criteria are on the Specification tab.\u003c\/li\u003e\n      \u003c\/ul\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e\n","brand":"ufcbio","offers":[{"title":"1X — Ready to use","offer_id":41999939502266,"sku":"BPBS74-1X","price":19.99,"currency_code":"USD","in_stock":true},{"title":"10X — Concentrated stock","offer_id":50665478095034,"sku":"BPBS74-10X","price":34.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0625\/9789\/0234\/files\/1X_PBS_70cb6d34-cb82-481f-b4f9-5a9781aae55c.webp?v=1775232977","url":"https:\/\/ufcbio.com\/products\/pbs-phosphate-buffered-saline","provider":"UFCBIO","version":"1.0","type":"link"}