MES Monohydrate CAS No: 145224-94-8

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MES also known as 4-Morpholinoethanesulfonic acid is used as a buffering agent in Life Sciences. It is a zwitterionic organic buffer and is one of the original Good’s buffers [Good et al. 1966].

Description

MES also known as 4-Morpholinoethanesulfonic acid is used as a buffering agent in Life Sciences. It is a zwitterionic organic buffer and is one of the original Good’s buffers [Good et al. 1966]. MES buffer has a pKa value of 6.10 at 25°C. It is very soluble in water, not absorbed through the cell membranes, almost inert in biochemical reactions, essentially transparent to visible and UV light, and chemically / ezymatically stable. It is widely used in cell culture media, electrophoresis, and molecular biology applications.

CAS Registry Number 145224-94-8
Name MES Monohydrate
Synonyms 2-(N-Morpholino)ethanesulfonic acid, 4-Morpholineethanesulfonic acid monohydrate
Molecular Formula C6H13NO4S4• H2O
Molecular Weight 213.25
Molecular Structure

Specification

Test M1101, High Purity M1103, Ultra Pure M1105, Ultra BioPure
Appearance White crystalline powder White crystalline powder White crystalline powder
Assay (by titration, on dried basis) ≥ 98.0% ≥ 99.0% ≥ 99.0%
Solubility (1M solution in H2O) Clear, complete and colorless Clear, complete and colorless Clear, complete and colorless
Identification (by IR) Conforms to structure Conforms to structure Conforms to structure
Loss on Drying (105°C for 3 hours) ≤ 9.5% ≤ 9.5% ≤ 9.5%
pH (0.5M solution in water) 2.5 – 4.0 2.5 – 4.0 2.5 – 4.0
pKa (@ 20ºC) 6.05 – 6.25 6.05 – 6.25 6.05 – 6.25
UV Absorption (1M @290nm) ≤ 0.05 ≤ 0.05
UV Absorption (1M @280nm) ≤ 0.05 ≤ 0.05
UV Absorption (1M @260nm) ≤ 0.05 ≤ 0.05
Residue on Ignition ≤ 0.10% ≤ 0.10%
Insoluble Matter ≤ 0.1% ≤ 0.1%
Heavy Metals as Lead ≤ 0.0015% ≤ 0.0015%
Biological Impurity Testing
Test M1101, High Purity M1103, Ultra Pure M1105, Ultra BioPure
Endotoxin (0.1% solution in H2O) ≤ 0.10 EU/mL
Bioburden ≤ 100 CFU/g
RNase, DNase, Nickase, Protease None detected
Trace Impurity Testing
Test M1101, High Purity M1103, Ultra Pure M1105, Ultra BioPure
Iron (Fe) ≤ 0.0005% ≤ 0.0005%
Chloride (Cl) ≤ 0.01% ≤ 0.01%
Sulfate (SO4) ≤ 0.05% ≤ 0.05%
Specification Sheet
Specification Sheet MES Monohydrate Spec MES Monohydrate Spec MES Monohydrate Spec

MSDS

Material Safety Data Sheet