HEPES has been described as one of the best all-purpose buffers available for biological research.1 Similar to many amino acids, HEPES is zwitterionic at most biological pHs. It’s effective as a buffer in the pH range of 6.8 to 8.2, and in concentrations between 10 millimolar to 25 millimolar. HEPES is one of the most effective buffers in the important physiological pH range of 7.2 to 7.6. HEPES has been utilized extensively for research and commercial applications in the cell culture and tissue culture industries. Other large application areas include cosmetics and skin care, electronics and computer chip manufacturing.
1 N.E. Good, et al., Biochemistry, 5, 467 (1966).
|CAS Registry Number||7365-45-9|
|Synonyms||4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid; N-2-Hydroxyethylpiperazine-N’-2-ethanesulfonic acid; 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid|