HEPES CAS No: 7365-45-9

Availability: In Stock

HEPES has been described as one of the best all-purpose buffers available for biological research.1 Similar to many amino acids, HEPES is zwitterionic at most biological pHs.

Description

HEPES has been described as one of the best all-purpose buffers available for biological research.1 Similar to many amino acids, HEPES is zwitterionic at most biological pHs. It’s effective as a buffer in the pH range of 6.8 to 8.2, and in concentrations between 10 millimolar to 25 millimolar. HEPES is one of the most effective buffers in the important physiological pH range of 7.2 to 7.6. HEPES has been utilized extensively for research and commercial applications in the cell culture and tissue culture industries. Other large application areas include cosmetics and skin care, electronics and computer chip manufacturing.

1 N.E. Good, et al., Biochemistry, 5, 467 (1966).

CAS Registry Number 7365-45-9
Name HEPES
Synonyms 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid; N-2-Hydroxyethylpiperazine-N’-2-ethanesulfonic acid; 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid
Molecular Formula C8H18N2O4S
Molecular Weight 238.30
Molecular Structure

Specification

 

Test H1108, High Purity H1104, Ultra Pure H1107, Ultra Biopure
Price
Request Quote
Request Quote
HEPES Price
Appearance White crysatlline powder White crystalline powder White crystalline powder
Assay (by titration, on dried basis) NLT 99.0% NLT 99.5% NLT 99.5%
Solubility (1M solution in H2O) Clear, complete and colorless Clear, complete and colorless Clear, complete and colorless
Identification (by IR) Acceptable Conforms to structure Conforms to structure
Melting Point
Water (105°C for 3 hours) ≤ 1.0% ≤ 1.0% ≤ 1.0%
Water (KF) ≤ 0.5% ≤ 0.5%
pH (5% solution in water) 5.0 – 6.5 5.0 – 6.5 5.0 – 6.5
pKa (@25°C) 7.45 – 7.65 7.45 – 7.65 7.45 – 7.65
UV Absorption (1M @290nm) ≤ 0.04 ≤ 0.04
UV Absorption (1M @280nm) ≤ 0.04 ≤ 0.05
UV Absorption (1M @260nm) ≤ 0.05 ≤ 0.05
Residue on Ignition ≤ 0.1% ≤ 0.1%
Insoluble Matter
Heavy Metals (Lead) ≤ 0.0005% ≤ 0.0005% ≤ 0.0005%
Biological Impurity Testing
Test H1108, High Purity H1104, Ultra Pure H1107, Ultra Biopure
Endotoxin NMT 0.1 EU/mg
Endotoxin (0.2% solution in H2O ≤ 0.10 EU/mL
Bioburden NMT 100 CFU/g
RNase, DNase, Nickase, Protease None detected
Cell culture test Pass
Trace Impurity Testing
Test H1108, High Purity H1104, Ultra Pure H1107, Ultra Biopure
Aluminum (Al)
Arsenic (As)
Barium (Ba)
Bismuth (Bi)
Cadmium (Cd)
Calcium (Ca)
Chloride (Cl)
Chromium (Cr)
Cobalt (Co)
Copper (Cu)
Iron (fe) ≤ 0.0005% ≤ 0.0005% ≤ 0.0005%
Lithium (Li)
Magnesium (Mg)
Manganese (Mn)
Molybdenium (Mo)
Nickel (Ni)
Potassium (K)
Sodium (Na)
Strontium (Sr)
Zinc (Zn)
Sulfate (S04) ≤ 0.05% ≤ 0.05% ≤ 0.05%
Specification Sheet
Specification Sheet HEPES Spec HEPES Spec HEPES Spec

MSDS

Material Safety Data Sheet