HEPES Sodium CAS No: 75277-39-3

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HEPES has been described as one of the best all-purpose buffers available for biological research.1 Similar to many amino acids, HEPES is zwitterionic at most biological pHs.

Description

HEPES has been described as one of the best all-purpose buffers available for biological research.1 Similar to many amino acids, HEPES is zwitterionic at most biological pHs. It’s effective as a buffer in the pH range of 6.8 to 8.2, and in concentrations between 10 millimolar to 25 millimolar. HEPES is one of the
most effective buffers in the important physiological pH range of 7.2 to 7.6. HEPES has been utilized extensively for research and commercial applications in the cell culture and tissue culture industries. Other large application areas include cosmetics and skin care, electronics and computer chip manufacturing.

1 N.E. Good, et al., Biochemistry, 5, 467 (1966).

CAS Registry Number 75277-39-3
Name HEPES Sodium Salt
Synonyms 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt
N-(2-Hydroxyethyl)piperazine-N’-(2-ethanesulfonic acid) sodium salt
Molecular Formula C8H17N2O4SNa
Molecular Weight 260.29
Molecular Structure

Specification

 

Test H1111, High Purity H1113, Ultra Pure H1115, Ultra BioPure
Appearance White powder White powder White powder
Assay (by titration, on dried basis) ≥ 98.0% ≥ 99.0% ≥ 99.0%
Solubility (1M solution in H2O) Clear, complete and colorless Clear, complete and colorless Clear, complete and colorless
Identification (by IR) Conforms to structure Conforms to structure Conforms to structure
Loss on Drying (105°C for 3 hours) ≤ 3.0% ≤ 3.0% ≤ 3.0%
pH (1% solution in water) 9.4 – 11.0 9.4 – 11.0 9.4 – 11.0
pKa (@ 25ºC) 7.4 – 7.7 7.4 – 7.7
UV Absorption (0.1M @290nm) ≤ 0.05 ≤ 0.05
UV Absorption (0.1M @280nm) ≤ 0.05 ≤ 0.05
UV Absorption (0.1M @260nm) ≤ 0.04 ≤ 0.04
Insoluble Matter ≤ 0.1% ≤ 0.1%
Heavy Metals (Lead) ≤ 0.0005% ≤ 0.0005%
Biological Impurity Testing
Test H1111, High Purity H1113, Ultra Pure H1115, Ultra BioPure
Endotoxin (0.1% solution in H2O) ≤ 0.04 EU/mL
Bioburden ≤ 100 CFU/g
Trace Impurity Testing
test H1111, High Purity H1113, Ultra Pure H1115, Ultra BioPure
Iron (Fe) ≤ 0.0005% ≤ 0.0005%
Specification Sheet
Specification Sheet HEPES Sodium Salt Spec HEPES Sodium Salt Spec HEPES Sodium Salt Spec

MSDS

Material Safety Data Sheet