HEPES Hemisodium CAS No: 103404-87-1

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HEPES has been described as one of the best all-purpose buffers available for biological research.1 Similar to many amino acids, HEPES is zwitterionic at most biological pHs.

Description

 

CAS Registry Number 103404-87-1
Name HEPES hemisodium salt
Synonyms 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid hemisodium salt, N-(2-Hydroxyethyl)piperazine-N’-(2-ethanesulfonic acid) hemisodium salt
Molecular Formula C8H17.5N2O4S • 0.5Na
Molecular Weight 249.30
Molecular Structure  

Specification

Test H1121, High Purity H1123, Ultra Pure H1125, Ultra BioPure
Appearance White powder White powder White crystalline powder
Assay (by titration, on dried basis) ≥ 98.0% ≥ 99.0% ≥ 99.0%
Solubility (1M solution in H2O) Clear, complete and colorless Clear, complete and colorless
Identification (by IR) Conforms to structure Conforms to structure Conforms to structure
Water (105°C for 3 hours) ≤ 3.0% ≤ 3.0% ≤ 3.0%
Water (KF) ≤ 2.0% ≤ 2.0%
pH (1% solution in water) 6.8 – 8.2 6.8 – 8.2 6.8 – 8.2
pKa (@ 25ºC) 7.4 – 7.7 7.4 – 7.7
UV Absorption (0.1M @290nm) ≤ 0.05 ≤ 0.05
UV Absorption (0.1M @280nm) ≤ 0.05 ≤ 0.05
UV Absorption (0.1M @260nm) ≤ 0.05 ≤ 0.05
Insoluble Matter ≤ 0.1% ≤ 0.1%
Heavy Metals (Lead) ≤ 0.0005% ≤ 0.0005%
Biological Impurity Testing
Test H1121, High Purity H1123, Ultra Pure H1125, Ultra BioPure
Endotoxin (0.1% solution in H2O) ≤ 0.1 EU/mL
Bioburden ≤ 100 CFU/g
Trace Impurity Testing
Test H1121, High Purity H1123, Ultra Pure H1125, Ultra BioPure
Iron (Fe) ≤ 0.0005% ≤ 0.0005%
Specification Sheet
Specification Sheet HEPES Hemisodium Spec HEPES Hemisodium Spec HEPES Hemisodium Spec

MSDS

Material Safety Data Sheet