Guanidine hydrochloride has proved to be the most powerful among commonly used protein denaturants, unfolding proteins. Highly concentrated (6 – 8 M) Guanidine HCl solutions are used to denature native globular proteins. It apparently disrupts hydrogen bonds which hold the protein in its unique structure.1 Guanidine HCl is used in the isolation of RNA to dissociate the nucleoprotein into its nucleic acid and protein moieties.2
Guanidine chloride is used in HPLC in-line sorption enhancement of xenobiotics in biological fluids.3 Guanidinium chloride is a chaotropic agent, a substance that disrupts the structure of water interactions to solubilize hydrophobic proteins / peptides.
Guanidine hydrochloride interferes with or inhibits the replication of many plant and animal viruses.4
Guanidine HCl specifically inhibits viral dsRNA synthesis while sparing both positive sense RNA synthesis and viral mRNA translation, allowing for the temporal separation of viral gene expression and genome replication.5
- Manufactured to meet the highest specifications
- Free from heavy metal contaminants
- Free from ammonia and other degradation products
- Free from UV absorbing materials in 230 to 300 nm
11972 Nozaki Y., The Preparation of Guanidine HCl, Methods in Enzymology, Enzyme Structures, Part C, vol. 26, pp. 43-50, 1972.
21968 Cox R. A., The Use of Guanidinium Chloride in the Isolation of Nucleic Acids, Methods in Enzymology, 12B, 120-9.
31993 P.B. Krugera, C.F., et al., Use of guanidine hydrochloride and ammonium sulfate in comprehensive in-line sorption enrichment of xenobiotics in biological fluids by high-performance liquid chromatography, Journal of Chromatography B: Biomedical Sciences and Applications, 612(2), 191-8.
41973, Caliguiri, L. A., and I. Tamm. Guanidine and 2-(-hydroxylbenzyl)-benzimidazole) (HBB): selective inhibitors of picornavirus multiplication, Selective inhibitors of viral function, W. Carter (ed.),. CRC Press, Inc., Cleveland, OH. 257–93.
52007, Murray, K.E, and M.L. Nibert, Guanidine Hydrochloride Inhibits Mammalian Orthoreovirus Growth by Reversibly Blocking the Synthesis of Double-Stranded RNA, Journal of Virology, 4572-84.
|CAS Registry Number||50-1-1|