Bis-Tris Propane CAS No: 64431-96-5

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Description

Bis-Tris propane is a zwitterionic buffer that is used in Life Sciences. It has a wide buffering range, from pH 6 to 9.5, due to its two distinct pKa values.

CAS Registry Number 64431-96-5
Name BIS-TRIS propane
Synonyms 1,3-Bis[tris(hydroxymethyl)methylamino]propane
Molecular Formula C11H26N2O6
Molecular Weight 282.33
Molecular Structure

Specification

Test B5501, High Purity B5503, Ultra Pure B5505, Ultra BioPure
Price
Appearance White powder White powder White powder
Assay (by titration, on dried basis) ≥ 98.0% ≥ 99.0% ≥ 99.0%
Tris Content ≤ 1.0% ≤ 1.0%
Solubility (1% solution in H2O) Clear, complete and colorless Clear, complete and colorless Clear, complete and colorless
Identification (by IR) Conforms to structure Conforms to structure Conforms to structure
Loss on Drying (110°C for 3 hours) ≤ 1.0% ≤ 1.0% ≤ 1.0%
Water (KF)
pH (1% solution in water) 10.0 – 12.0 10.0 – 12.0 10.0 – 12.0
UV Absorption (1M @280nm) ≤ 0.05 ≤ 0.05
UV Absorption (1M @260nm) ≤ 0.10 ≤ 0.10
Residue on Ignition ≤ 0.10% ≤ 0.10%
Insoluble Matter ≤ 0.10% ≤ 0.10%
Heavy Metals as Lead ≤ 0.0005% ≤ 0.0005%
Biological Impurity Testing
Test B5501, High Purity B5503, Ultra Pure B5505, Ultra BioPure
Endotoxin (0.1% solution in H2O) ≤ 0.1 EU/mL
Bioburden ≤ 100 CFU/g
Trace Impurity Testing
Test B5501, High Purity B5503, Ultra Pure B5505, Ultra BioPure
Iron (Fe) ≤ 0.0005% ≤ 0.0005%
Specification Sheet
Specification Sheet Bis-Tris Propane Spec Bis-Tris Propane Spec Bis-Tris Propane Spec

Related

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MSDS

Material Safety Data Sheet

Application

Bis-Tris propane has been used in protein crystallization.1
Bis-Tris popane is a useful buffer for PCR.2
The preparation and phosphodiester hydrolysis activity of complexes of lanthanum and Bis-Tris propane have been investigated.3
Bis-Tris Propane as a New Polydentate Linker in the Synthesis of Iron (lll) and Manganese (ll/lll) Complexes.4
11997 Knapp S., et al., Crystal structure of glutamate dehydrogenase from the hyperthermophilic eubacterium Thermotoga maritima at 3.0 Å resolution. J. Mol. Biol., 267(4), 916-32.
21991 Eckert K. A., and Kunkel T. A., DNA polymerase fidelity and the polymerase chain reaction, PCR Methods Appl., 1(1), 17-24.
32001 Gomez-Tagle P., and Yatsimirsky A. K., Phosphodiester hydrolysis by lanthanide complexes of bis-tris propane. Inorg. Chem., 40(15), 3786-96.
42008 Ferguson A., et al., Bis-Tris Propane as a New Polydentate Linker in the Synthesis of Iron(III) and Manganese(II/III) Complexes, Inorganic Chemistry, 47(21), 4742-44.

Product Highlights

Manufactured to meet the highest specifications.
Free from heavy metal contaminants.
Free from starting materials and degradation products.
Free from UV absorbing materials in 230 to 300 nm.